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  • Title: Interferon-alpha yields from Sendai virus-induced human leukocyte cultures are enhanced by lowering the incubation temperature.
    Author: Stecher A, Mohr H, Schügerl K.
    Journal: J Gen Virol; 1984 Mar; 65 ( Pt 3)():635-8. PubMed ID: 6321641.
    Abstract:
    Increased yields of Sendai virus-induced human leukocyte interferon (IFN-alpha) were obtained by lowering the incubation temperature from the initial 37 degrees C within 1 to 6 h after the addition of virus. Maximal stimulation was found when this was done 2 to 4 h after induction and when the lowered temperature was between 29 and 32 degrees C. Compared to cultures kept at 37 degrees C throughout, the resulting IFN-alpha yields were enhanced by 2.5- to 3-fold. This was mainly due to prolonged synthesis of IFN-alpha, which could be observed up to 20 h after induction, whereas in cultures kept at 37 degrees C the production of IFN-alpha had practically ceased approx. 10 h after the addition of virus.
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