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  • Title: DNA methylation inhibits the transfecting activity of replicative- form phi X174 DNA.
    Author: Wang RY, Shenoy S, Ehrlich M.
    Journal: J Virol; 1984 Mar; 49(3):674-9. PubMed ID: 6321756.
    Abstract:
    Replacement of virtually all the cytosine residues with 5-methylcytosine residues in the complementary strand of the replicative form (RF) of phi X174 DNA caused a 300- to 500-fold loss in its transfecting activity. Similar results were obtained with analogously methylated M13 RF. Transfection experiments with phi X RF hemimethylated in only part of the molecule, as assessed by analysis with restriction endonucleases, indicated that gene A of phi X, which needs to be nicked at a specific site by the gene A protein for RF replication, was not the main target for this inhibition by DNA methylation. We propose that the loss of transfecting activity was due to hemimethylation of the phi X RF interfering with the processively catalyzed movement of the replication fork.
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