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  • Title: Pancreatic CCK receptors: characterization of covalently labeled subunits.
    Author: Sakamoto C, Goldfine ID, Williams JA.
    Journal: Biochem Biophys Res Commun; 1984 Jan 30; 118(2):623-8. PubMed ID: 6322755.
    Abstract:
    125I-CCK was crosslinked with ultraviolet light to its receptor on pancreatic plasma membranes. The predominant labeled species following polyacrylamide gel electrophoresis had a molecular weight of 120,000 in the absence, and 80,000 in the presence of the reducing agent dithiothreitol. The M = 120,000 labeled band could be extracted, reduced and converted to Mr = 80,000. Moreover, peptide mapping with Staph aureus V8 protease showed a similar pattern for the 120,000 and 80,000 dalton bands. The crosslinked receptor could be solubilized with Triton X-100, absorbed to wheat germ agglutinin and eluted with N-acetylglucosamine. The results indicate, therefore, that the CCK receptor is a glycoprotein with subunits coupled by disulfide bonds.
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