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  • Title: Negatively supercoiled DNA from plants infected with a single-stranded DNA virus.
    Author: Sunter G, Coutts RH, Buck KW.
    Journal: Biochem Biophys Res Commun; 1984 Feb 14; 118(3):747-52. PubMed ID: 6322762.
    Abstract:
    A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of -0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme.
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