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  • Title: Extraction of SV40 T-antigen from lysates of transformed cells.
    Author: Lenstra JA, Smits RA, Bloemendal H.
    Journal: FEBS Lett; 1984 Mar 12; 168(1):129-33. PubMed ID: 6323217.
    Abstract:
    Subcellular fractions from SV-40 transformed hamster lens cells, prepared by chemical extractions, were tested for the presence of T-antigen by immunoautoradiography. Most of the T-antigen was present in the nucleus and was resistant to extraction by 2 M NaCl, indicating an association with the nuclear matrix. Another part of the T-antigen was, under certain conditions, resistant to extraction of the cells with a nonionic detergent. This T-antigen could be solubilized by Ca2+ at low temperature, conditions that also cause a specific depolymerization of microtubules.
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