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  • Title: The genes for the ribosomal proteins S12 and S7 are clustered with the gene for the EF-Tu protein on the chloroplast genome of Euglena gracilis.
    Author: Montandon PE, Stutz E.
    Journal: Nucleic Acids Res; 1984 Mar 26; 12(6):2851-9. PubMed ID: 6324129.
    Abstract:
    We characterize a DNA segment of the Euglena gracilis chloroplast DNA fragment Eco . N by nucleotide sequencing and S1 nuclease analysis. We show that this region, which is upstream of the previously sequenced tuf A gene, contains the genes for the ribosomal proteins S12 and S7. The gene arrangement is 5'-rps 12-80 bp spacer-rps 7-174 bp spacer-tuf A, somewhat similar to the str operon of E. coli. The chloroplast S12 and S7 proteins contain 124 and 155 aminoacids, respectively, and are to 68% and 38% homologous with the corresponding E. coli proteins. The region is transcribed into a distronic mRNA of about 1.1 to 1.2 kb. The rps 12 and rps 7 genes, contrary to the tuf A gene, are not split.
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