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  • Title: Subcellular localization of neutral red staining in Limulus ventral photoreceptors.
    Author: Chamberlain SC, Battelle BA, Calman BG.
    Journal: J Neurobiol; 1984 Mar; 15(2):79-87. PubMed ID: 6325595.
    Abstract:
    Limulus ventral photoreceptors are vitally stained by neutral red. In other systems such staining has been correlated with the presence of monoamines or neuropeptides. The stained cellular components in ventral photoreceptors are clusters of small ovoids which have been identified as residual bodies. These structures are unlikely candidates for monoamine or neuropeptide synthesis or storage sites, but may be part of the cyclic synthesis and degradation of photosensitive membrane. While vital staining with neutral red is a particularly useful method for identifying certain classes of neurons in vivo, in the case of ventral photoreceptors, the association of the vital staining property with the presence of a particular class of neurotransmitter candidates has proven difficult. Neutral red is useful, however, for visualizing the segmentation of ventral photoreceptors in vivo.
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