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  • Title: Preparation of ligation intermediates and related polynucleotide pyrophosphates.
    Author: Chu BC, Orgel LE.
    Journal: Biochim Biophys Acta; 1984 May 15; 782(1):103-5. PubMed ID: 6326832.
    Abstract:
    Unprotected oligonucleotides and oligodeoxynucleotides terminated with an unhindered 5'-phosphate group react with nucleoside 5'- phosphorimidazolides in aqueous solution to give 'capped' pyrophosphates in at least 70% yield. If adenosine 5'- phosphorimidazolide is used as a substrate in the reaction, ligase intermediates are obtained as products.
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