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  • Title: Molecular cloning and restriction mapping of a simian virus 40 deletion mutant derived from simian transformants expressing a non-karyophilic T antigen.
    Author: Fantuzzi LF, Vesco C.
    Journal: FEBS Lett; 1984 May 07; 170(1):125-30. PubMed ID: 6327371.
    Abstract:
    A replication-defective Simian virus 40 genome, with a deletion of about 120 nucleotides in the region encoding the N-terminal fourth of the large T antigen, has been isolated from the DNA of Simian cells transformed by SV40. Both the original transformants, and the murine transformants obtained by transfection with this cloned mutant DNA, produced a large T antigen displaying in immunofluorescence an exclusively cytoplasmic localization. The protein apparent molecular mass (83 kDa) was about 6% smaller than that of normal karyophilic large T. Restriction analysis showed that the deletion eliminated two close HinfI sites, at nucleotides 4459 and 4376 (map unit 0.50).
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