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  • Title: [Daughter DNA synthesis in UV-resistant and UV-sensitive Chinese hamster clones cells under UV irradiation].
    Author: Barenfel'd LS.
    Journal: Tsitologiia; 1984 Mar; 26(3):343-8. PubMed ID: 6328712.
    Abstract:
    By means of ultracentrifugation in alkaline sucrose gradients it has been shown that the size of DNA fragments synthesized in Chinese hamster cells of UV-sensitive clone (CHS-1) after exposure to UV light was equal to the distance between pyrimidine dimers in the parental DNA determined using endonuclease of Micrococcus luteus. With the UV-resistant clone (V-79), the length of fragments of the newly synthesized DNA was much longer than that between pyrimidine dimers in the parental DNA. The data obtained support the model according which DNA synthesis on the UV-irradiated template gives rise to gaps opposite to pyrimidine dimers.
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