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  • Title: Effect of different collagenases on the isolation of viable hepatocytes from rat liver.
    Author: Queral AE, DeAngelo AB, Garrett CT.
    Journal: Anal Biochem; 1984 Apr; 138(1):235-7. PubMed ID: 6329035.
    Abstract:
    The isolation of viable hepatocytes from rat liver was found to depend on the source of collagenase (EC 3.4.24.3) more than any other single factor examined. Collagenase purified from Achromobacter iophagus/Bacillus polymyxa (collagenase/dispase) gave reproducibly high viability without the use of complex perfusion protocols.
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