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  • Title: Chondrogenesis of chick limb mesenchyme in vitro. Effects of prostaglandins on cyclic AMP.
    Author: Biddulph DM, Sawyer LM, Smales WP.
    Journal: Exp Cell Res; 1984 Jul; 153(1):270-4. PubMed ID: 6329795.
    Abstract:
    The effects of prostaglandin E2 (PGE2) on cyclic AMP (cAMP) levels of chick limb bud cells during various stages of chondrogenesis were studied utilizing high density, micro-mass, cell culture. Concentrations of PGE2 in cell cultures at these same stages were measured by radio-immunoassay. Both basal levels of cAMP, as well as PGE2-stimulated changes in cAMP, increased maximally during the first 3 days of culture; this increase was associated with the formation of cell aggregates. Concentrations of PGE2 were also highest during this period. By 6 days of culture, nodules, containing cartilage matrix components, predominated. Both basal levels of cAMP and PGE2-stimulated cAMP levels were significantly decreased at this stage, relative to cultures at day 3. Concentrations of PGE2 fell dramatically in the 6-day cultures containing differentiated cartilage. These results support a regulatory role for both PGE2 and cAMP in the early events associated with chondrogenesis.
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