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  • Title: Cytochrome b561 catalyzes transmembrane electron transfer.
    Author: Srivastava M, Duong LT, Fleming PJ.
    Journal: J Biol Chem; 1984 Jul 10; 259(13):8072-5. PubMed ID: 6330096.
    Abstract:
    Purified cytochrome b561 from bovine adrenal medulla chromaffin vesicles has been reconstituted into phosphatidylcholine vesicles by a detergent-dialysis method. When the reconstituted cytochrome-containing vesicles were preloaded with ascorbic acid and cytochrome c was added to the external medium, the internal ascorbic acid was able to reduce the external cytochrome c. This reduction of cytochrome c was dependent on the presence of cytochrome b561 in the membrane and was not due to leakage of ascorbate from the vesicles. These results demonstrate that cytochrome b561 catalyzes a transmembrane electron transfer.
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