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  • Title: Two-dimensional restriction mapping by digestion with restriction endonucleases of DNA in agarose and polyacrylamide gels.
    Author: Boehm TL, Drahovsky D.
    Journal: J Biochem Biophys Methods; 1984 May; 9(2):153-61. PubMed ID: 6330196.
    Abstract:
    We have studied with a number of bacterial restriction enzymes the conditions for digestion of DNA in agarose and polyacrylamide gels. The restriction endonucleases HpaII, MspI, HaeIII, HindIII, TaqI, HhaI, AluI, BamHI, EcoRI and SalI are capable of digesting DNA in agarose gels of low electroendosmosis and low sulfate concentration. All enzymes, except BamHI, are also capable of digesting DNA in polyacrylamide gels. With this method, rapid two-dimensional restriction mapping of genomes with low and high sequence complexity is possible.
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