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  • Title: DNA restriction endonuclease analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG.
    Author: Collins DM, De Lisle GW.
    Journal: J Gen Microbiol; 1984 Apr; 130(4):1019-21. PubMed ID: 6330285.
    Abstract:
    DNA preparations from two reference (H37Ra and H37Rv) and two wild strains of Mycobacterium tuberculosis and one re-isolated strain of Mycobacterium bovis BCG were analysed using 17 restriction endonucleases. The enzyme BstEII revealed the greatest differences between strains. Electrophoretic DNA patterns from the wild M. tuberculosis strains differed from each other and from the reference strains at relatively few positions. At the highest resolution attained, patterns from the two reference strains remained indistinguishable from each other. The pattern of the M. bovis BCG strain was substantially different from, but had many bands in common with, the M. tuberculosis patterns.
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