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  • Title: [Exotoxin A production during Pseudomonas aeruginosa PA-7 cultivation in Martin's broth].
    Author: Brodinova NS, Baskakova NV, Moroz AF, Vertiev IuV, Mokrievich NM.
    Journal: Zh Mikrobiol Epidemiol Immunobiol; 1984 Apr; (4):22-6. PubMed ID: 6331028.
    Abstract:
    The activity of exotoxin A in culture filtrates prepared from cultures obtained by growing P. aeruginosa strains PA-7 and PA-103 in Martin's broth containing iron at a concentration of 0.08 microgram/ml, 0,05 M sodium glutamate and 1% of glycerin has been shown to be 1.5 times higher than that in filtrates prepared from cultures obtained by growing the above strains in a medium containing soybean tryptic digestion (USA). The optimun conditions for the production of exotoxin A by these strains are achieved during their cultivation in a fermenter at a temperature of 32 degrees C for 18 hours with simultaneous stirring (800 r. p. m.) and oxygenation (450 m3/h). Under these conditions the biological activity of the filtrates is 200 LD50/ml, their ADP-ribosyltransferase activity is 9500 c. p. m. and a sharply defined precipitation line appears in the double diffusion test in gel with monospecific antiserum to purified toxin, used in a dilution of 1:8.
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