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  • Title: Antibody and complement-mediated cytotoxicity for bovine parainfluenza-3 virus-infected cells.
    Author: Hussain A, Mohanty SB.
    Journal: Am J Vet Res; 1984 Jun; 45(6):1219-21. PubMed ID: 6331235.
    Abstract:
    An antibody-complement-mediated cytotoxicity assay was employed to determine whether susceptible host cells infected with bovine parainfluenza-3 virus could be destroyed by the humoral immune mechanism. The cytotoxicity assay yielded 21.5% to 76.9% specific release of 51Cr with sera collected from calves 28 days after inoculation with the virus. A multiplicity of infection of 5, a postinfection period of 2 days, an antibody dilution of 1:10, and an incubation period of 12 hours with antibody and complement were found to be optimal for this assay.
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