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  • Title: Ghost cells as sorption matrix for virus concentration from water.
    Author: Armon R, Kott Y, Neeman I.
    Journal: Appl Environ Microbiol; 1984 Jun; 47(6):1337-40. PubMed ID: 6331315.
    Abstract:
    Pig erythrocyte membranes were used as adsorbent material for the concentration of bacteriophage phi x-174, MS-2, and f2 from 5 ml of saline solution. The adsorption was carried out at pH 3.5, and the elution was carried out at pH 7.0. Compared with adsorption on 3% beef extract, bacteriophage adsorption on erythrocyte ghost cells yielded 93 to 100%, and elution was 92 to 100%, whereas beef extract organic flocculation yielded adsorption of 0 to 98.8% and elution of 61 to 86.6%. The same procedure but with poliovirus LSC-1 gave 100% adsorption and 91 to 129% elution.
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