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  • Title: Adenine nucleotide levels and adenosine metabolism in cultured calvarial bone.
    Author: Fredholm BB, Lerner U.
    Journal: Acta Physiol Scand; 1984 Apr; 120(4):551-5. PubMed ID: 6333138.
    Abstract:
    Adenine nucleotide levels were measured in extracts of murine calvaria after different periods of culture with or without parathyroid hormone (PTH; 10(-8) M) or PGE2 (10(-7) M). In control calvaria the energy charge, (ATP + 1/2 ADP)/(ATP + ADP + AMP), remained at close to 0.7 over a 24 hour culture period. However, bones cultured with PTH or PGE2 showed a transient fall in the energy charge down to 0.5. This was not associated with a fall in total adenine nucleotides. The rate of adenosine metabolism in cultured bone in vitro was studied by determining the contents of adenosine, inosine, 2-deoxyadenosine, 2-deoxyinosine and hypoxanthine in the culture medium. There was a continuous increase in adenosine, inosine and hypoxanthine as well as a disappearance of medium 2-deoxyadenosine that was accounted for by appearance of 2-deoxyinosine. The deaminating activity could only partly be accounted for by activity in the medium and thus probably mainly resides in the bone cells. PTH (10(-8) M) did not alter the rate of disappearance of 2-deoxyadenosine or adenosine deaminase activity determined in bone extracts. The results demonstrate that two substances that increase calcium mobilization from bone alter ATP utilization and/or synthesis without significantly influencing adenosine production or metabolism.
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