These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The detection of circulating immune complexes containing C1q and IgG using a new rapid serum ELISA test system.
    Author: Reckel RP, Harris J, Botsko E, Wellerson R, Varga S.
    Journal: Diagn Immunol; 1984; 2(4):228-37. PubMed ID: 6335421.
    Abstract:
    An ELISA sandwich assay for detecting circulating immune complexes (CIC) was evaluated. Heat-aggregated IgG (HAG) containing Clq (HAG:Clq) is used to generate standard curves for quantitation of CIC. Sera from 488 normal donors had an means of 3.8 +/- 15.3 micrograms/ml HAG eq and 97.1% had concentrations less than 35 micrograms/ml HAG eq (2 SD greater than means) and were considered negative. Sera from rheumatoid arthritis (RA) patients in active disease tested 86% positive for CIC (means = 104.5 +/- 63.4 micrograms/ml HAG eq) while 100% with inactive disease were negative (means = 11.4 +/- 3.7 micrograms/ml HAG eq). Similarly, in systemic lupus erythematosus (SLE) patients with active disease, 100% were positive (means = 133.1 +/- 50.6 micrograms/ml HAG eq) while 80% with inactive disease were negative (means = 20.4 +/- 21.4 micrograms/ml HAG eq). In AIDS (AIDS related), 88.5% of AIDS sera and 77.1% of lymphadenopathy syndrome (LAS) sera had elevated CIC. Sera from asymptomatic homosexual controls showed 85% positive for CIC. The serum ELISA test for CIC compared favorably with the 125I-staphylococcus binding assay but was more sensitive than the 125I-C1qBA. The OKT4/OKT8 ratios were also significantly reduced in AIDS and LAS.
    [Abstract] [Full Text] [Related] [New Search]