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  • Title: Proteolysis of acinetobacter citrate synthase by subtilisin.
    Author: Mitchell CG, Weitzman PD.
    Journal: FEBS Lett; 1983 Jan 24; 151(2):265-8. PubMed ID: 6339274.
    Abstract:
    Citrate synthase from Acinetobacter calcoaceticus was subjected to proteolysis with subtilisin. Although the enzyme proved relatively resistant to inactivation by this treatment, SDS-polyacrylamide gel electrophoresis clearly revealed breakdown of the citrate synthase to smaller fragments. The regulatory responses of the native enzyme to inhibition by NADH and re-activation by AMP were retained on proteolysis, indicating that the fragments bind tightly to each other and preserve the overall cooperative molecular interactions.
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