These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Binding of lac repressor headpiece to poly[d(A-T)]. A thermal denaturation study.
    Author: Durand M, Schnarr M, Maurizot JC.
    Journal: Biochem Biophys Res Commun; 1983 Jan 14; 110(1):169-75. PubMed ID: 6340664.
    Abstract:
    The binding of the lac repressor headpiece to poly[d(A-T)] has been investigated using thermal denaturation experiments. The binding of the headpiece leads to a stabilization of the double-stranded structure. Competition experiments using circular dichroism measurements confirm that the headpiece binds stronger on the double-stranded poly[d(A-T)] than on a single-stranded nucleic acid like poly(A). Theoretical analyses of the melting curves allow the determination of the binding constant and of the site size on the poly[d(A-T)]. This latter value is found to be 4 base pairs, in good agreement with that determined by other experimental approaches, and is much smaller than the values previously found for the lac repressor.
    [Abstract] [Full Text] [Related] [New Search]