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  • Title: [Increased yields of insulin by recycling of non-combined insulin chains].
    Author: Schartmann B, Gattner HG, Danho W, Zahn H.
    Journal: Hoppe Seylers Z Physiol Chem; 1983 Feb; 364(2):179-86. PubMed ID: 6341201.
    Abstract:
    The yield of insulin by the combination of reduced A and B chains is increased from 15% to 33-34% if the non-insulin-containing fractions of the gel filtration are isolated and used for further combinations (recycling). Crude insulin recovered by gel filtration can be highly purified by preparative high-performance liquid chromatography. Lower yields of insulin were obtained on recombination of chains which had been recycled five times or more. Sulfur analyses show a loss of cystine sulfur. The occurrance of lanthionine and lysinoalanine indicates an alkaline destruction of cystine/cysteine during the repeated combination steps.
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