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  • Title: Purification of DNA-dependent RNA polymerase II from Saccharomyces cerevisiae.
    Author: Bitter GA.
    Journal: Anal Biochem; 1983 Feb 01; 128(2):294-301. PubMed ID: 6342468.
    Abstract:
    A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, and chromatography on DEAE-cellulose, DEAE-Sephadex, and phosphocellulose. The procedure may be completed in 2.5 days and the resultant enzyme is judged to be greater than 90% pure.
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