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  • Title: Amplification of D-xylose and D-glucose isomerase activities in Escherichia coli by gene cloning.
    Author: Wovcha MG, Steuerwald DL, Brooks KE.
    Journal: Appl Environ Microbiol; 1983 Apr; 45(4):1402-4. PubMed ID: 6344793.
    Abstract:
    A recombinant plasmid, designated pUC1002, was constructed by ligation of a HindIII restriction endonuclease fragment of Escherichia coli chromosomal DNA to vector plasmid pMB9. Strains carrying this plasmid were selected by transformation of an E. coli strain bearing the xyl-7 mutation to a xylose-positive (Xyl+) phenotype. Strains containing pUC1002 produced coordinately elevated levels of D-xylose isomerase and D-xylulose kinase. Under appropriate conditions, the isomerase also efficiently catalyzed the conversion of D-glucose to D-fructose.
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