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  • Title: [Purification of the extracellular trypsin inhibitor from Actinomyces janthinus 118].
    Author: Andreeva NA, Chermenskiĭ DN, Bezborodov AM.
    Journal: Prikl Biokhim Mikrobiol; 1978; 14(1):25-31. PubMed ID: 634933.
    Abstract:
    From the culture liquid filtrate of Actinomyces janthinus 118 a trypsin inhibitor (TI-AjI) was isolated by sulphate ammonium precipitation, DEAE-cellulose chromatography, QAE-Sephadex chromatography and two-fold crystallization. The resultant preparation was homogeneous during polyacrylamide gel electrophoresis and ultracentrifugation. As measured by gel filtration, the TI-AjI molecular weight was estimated to be 13,000+/-1,000. The culture liquid of Act. janthinus 118 was found to contain another substance with anti-trypsin activity that differed from TI-AjI in its molecular weight.
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