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  • Title: [Interaction between immobilized plastocyanin and cytochrome f and the photosystem I reaction center in a reconstituted system].
    Author: Khristin MS.
    Journal: Biokhimiia; 1983 Jul; 48(7):1067-73. PubMed ID: 6351934.
    Abstract:
    The effects of redox conversions of plastocyanin copper chromophore on the formation of plastocyanin complexes with cytochrome f and the reaction center of photosystem I from pea chloroplasts were studied. In order to investigate the complex formation plastocyanin and cytochrome f were immobilized on Sephadex G-200. The cytochrome f and reaction center assembly takes place on the immobilized plastocyanin, which is necessary for cytochrome f photooxidation. It was found that in a reconstituted system the reduced plastocyanin forms more stable complexes with the proteins than the oxidized one, which is due to its lower pI value.
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