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  • Title: [Determination of the activity of a polynucleotide phosphorylase preparation from E. coli].
    Author: Pupkova VI, Raspopina GI.
    Journal: Prikl Biokhim Mikrobiol; 1983; 19(4):555-9. PubMed ID: 6353407.
    Abstract:
    Thin-layer chromatography was employed to measure the activity of polynucleotide phosphorylase (PNP-ase) from E. coli during purification and in the resultant preparation. The TLC data were verified with respect to released inorganic orthophosphate and to 14C-AMP incorporation rate. It is concluded that TLC can be used to determine PNP-ase activity in crude extract, at various purification stages and in the final preparation, yielding correct results.
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