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  • Title: [Assay of rheumatoid factors by the indirect immunofluorescence method].
    Author: Ruffatti A, De Silvestro G, Meneghini P, Doria A, Volante D, Gambari PF, Todesco S.
    Journal: Boll Soc Ital Biol Sper; 1983 Aug 30; 59(8):1156-9. PubMed ID: 6354210.
    Abstract:
    Sera from 69 patients affected with rheumatoid arthritis were examined for IgM, IgG and IgA rheumatoid factors (RF) by a indirect immunofluorescence method. The results were compared with those obtained from the classical rheumatoid factor latex test. By this technique we have demonstrated antigammaglobulin activity in a high proportion (23%) of sera from latex test seronegative rheumatoid patients. Moreover, by fractionated antisera it was possible to detect also IgG and IgA factors. Indirect immunofluorescence results to be a simple and available technique for detection of RF, also in many "seronegative" patients.
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