These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Cycloheximide causes accumulation of insulin receptors at the cell surface of cultured fibroblasts.
    Author: Kadle R, Kalter VG, Raizada MK, Fellows RE.
    Journal: J Biol Chem; 1983 Nov 10; 258(21):13116-9. PubMed ID: 6355103.
    Abstract:
    Incubation of confluent cultures of mouse fibroblasts with cycloheximide caused a time-dependent increase in the binding of 125I-insulin. The increase was concentration-dependent between 0.05 and 3.5 microM cycloheximide and showed a high correlation (r = 0.97) between the increase in 125I-insulin binding and the inhibition of protein synthesis. In the presence of 3.5 microM cycloheximide, insulin binding increased to 236% of control and incorporation of [3H]valine into proteins fell to 10-20% of control. Scatchard analysis of the binding data indicated that cycloheximide-treated cultures had a total of 6.6 X 10(4) binding sites/cell compared to 3.9 X 10(4) sites/cell in untreated cultures. No significant changes in affinity were observed. Other protein synthesis inhibitors also caused an increase in 125I-insulin binding. With 25 mM ethionine and 2 mM sodium fluoride, binding was 155 and 245% of control and incorporation of [3H]leucine into proteins was decreased to 41 and 47% of control, respectively. These results suggest that the accumulation of insulin receptors at the cell surface following treatment with cycloheximide results from inhibition of synthesis of proteins involved in insulin receptor turnover.
    [Abstract] [Full Text] [Related] [New Search]