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  • Title: Cloning and deletion mapping of the recF dnaN region of the Escherichia coli chromosome.
    Author: Ream LW, Clark AJ.
    Journal: Plasmid; 1983 Sep; 10(2):101-10. PubMed ID: 6356186.
    Abstract:
    By cloning a 3.6-kb EcoRI fragment of the Escherichia coli chromosome with pBR322 we located more precisely recF relative to dnaN. By deletion mapping we localized functional recF to a 1.65-kb region of the cloned fragment and allowed rough mapping of the C terminus of dnaN. Cloned recF+, separated from functional flanking genes dnaN and gyrB, complemented chromosomal recF mutations presumably by coding for a cytodiffusible product. The protein encoded by dnaN was observed as a band on a polyacrylamide gel from minicells. Identification of a recF protein was not made.
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