These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Insulin dissociation from its receptors on human fat cells. Evaluation of the negative cooperativity model.
    Author: Arner P, Bolinder J, Ostman J.
    Journal: Acta Diabetol Lat; 1983; 20(3):197-203. PubMed ID: 6356734.
    Abstract:
    Insulin dissociation from its receptors on isolated human fat cells was investigated using mono-125I-[Tyr A 14]-insulin. Fat cells were equilibrated with 0.05 pmol/ml of the radioligand and then transferred to a radioactive free buffer. The rate of dilution-induced dissociation of bound labelled insulin was enhanced in the presence of native insulin (1.7 nmol/ml), both at 24 degrees C and 37 degrees C and in both omental and subcutaneous adipocytes. The dissociation of radioactive insulin was more rapid when fat cells were equilibrated with 0.15 than with 0.05 pmol/ml of radioactive insulin, both in the presence or absence of an excess of native insulin. Thus, the presence of site-site interactions of the negative cooperativity type among insulin receptors of human fat cells is demonstrated.
    [Abstract] [Full Text] [Related] [New Search]