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  • Title: Identification of bacteriophage T4D gene 29 product, a baseplate hub component, as a folylpolyglutamate synthetase.
    Author: Sadewasser DA, Kozloff LM.
    Journal: Biochem Biophys Res Commun; 1983 Nov 15; 116(3):1119-24. PubMed ID: 6360172.
    Abstract:
    An assay for folylpolyglutamate synthetase activity in extracts of uninfected and bacteriophage T4D-infected Escherichia coli B has been developed. T4D infection induced the formation of a new synthetase raising the total synthetase activity three-fold. Extracts obtained after infection with T4 gene 51, 27 or 28 amber mutants showed increased synthetase activities while extracts obtained from cells infected with a T4D gene 29 amber mutant did not show any increase in synthetase activity. The phage-induced synthetase was found to copurify with the gene 29 product and a 100-fold purified synthetase of molecular size of 74,000 daltons has been obtained. The purified synthetase has a folate substrate specificity different from the host synthetase since it added glutamate residues to dihydrofolate as well as to the usual tetrahydrofolate substrate.
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