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  • Title: Hydrolysis of phenylthiazolones of p-guanidinophenylalanine and arginine by trypsin and related enzymes.
    Author: Tsunematsu H, Hatanaka Y, Sugahara Y, Makisumi S.
    Journal: J Biochem; 1983 Oct; 94(4):1119-25. PubMed ID: 6361008.
    Abstract:
    The phenylthiazolone (PTA) of p-guanidinophenylalanine (GPA) was synthesized and the susceptibility of this compound to trypsin and related enzymes was compared with that of the PTA of arginine (Arg). Both PTA-GPA and PTA-Arg were almost completely and rapidly hydrolyzed by trypsin and pronase. PTA-Arg was hydrolyzed rapidly by thrombin, whereas PTA-GPA was less susceptible to this enzyme. The rates of hydrolysis of the two PTAs by alpha-chymotrypsin and papain were fairly slow. The specificity constant (kcat/Km) for the hydrolysis of PTA-GPA by trypsin was about 4 times larger than that of PTA-Arg. Thus, PTA-GPA, as well as PTA-Arg, behaves as a specific internal thioester substrate for trypsin and is the best one in the series of PTA substrates examined so far. However, PTA derivatives of GPA and Arg were hydrolyzed with kcat/Km values smaller than those of N alpha-benzoyl ethyl esters of L-GPA and L-Arg by factors of 4 and 17, respectively.
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