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  • Title: Comparison of ribosomal proteins of chloroplast from spinach and of E. coli.
    Author: Dorne AM, Eneas-Filho J, Heizmann P, Mache R.
    Journal: Mol Gen Genet; 1984; 193(1):129-34. PubMed ID: 6361495.
    Abstract:
    A comparison of ribosomal proteins from Escherichia coli and from chloroplasts of Spinach was made using two separate methods: electrophoretic migration and immunochemical cross-reaction between blotted E. coli ribosomal proteins and chloroplast ribosomal subunits antisera. It is shown that L2 from E. coli (E-L2) and L4 from chloroplasts (CS-L4) comigrated and that E-L2 immunologically cross-reacted with the isolated CS-L4 antibody. Co-migration was observed for three additional couples of 50S ribosomal proteins. It is also shown that at least one 30S E. coli ribosomal protein immuno-cross reacted with a 30S chloroplast antiserum and that three couples of 30S ribosomal proteins comigrated.
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