These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Receptor-mediated 125I-labelled insulin degradation in the rat hepatocyte. Studies using chloroquine.
    Author: Bonser AM, Garcia-Webb P, Bhagat CI.
    Journal: Aust J Exp Biol Med Sci; 1983 Oct; 61 ( Pt 5)():569-79. PubMed ID: 6362640.
    Abstract:
    Degradation of insulin during incubation with target cells occurs via receptor-mediated processes. In this study, receptor-mediated degradation of 125I-labelled insulin was investigated in rat hepatocytes, using the agent chloroquine. Chloroquine increased specific cell-associated 125I-labelled insulin at 37 degrees. The increased radioactivity with chloroquine was intracellular (53.3 +/- 1.2% of initially bound label was displaced by excess cold insulin; in control cells 67.0 +/- 2.1% was displaced, P less than 0.005). The effect of chloroquine was prevented by adding label at 15 degrees or by pre-treatment with 5 mM KCN, 5 mM NaN3 or 1 g/l bacitracin, which indicated a post-internalization site of action. Chloroquine had no effect on degradation of 125I-labelled insulin in buffer alone or in buffer previously incubated with cells. Specific studies of receptor-mediated degradation at 37 degrees showed that more 125I-labelled insulin remained associated with hepatocytes when chloroquine was present (P less than 0.0005 after 60 min). Analysis of chloroquine's effect on the intactness of 125I-labelled insulin released during processing of surface-bound label at 37 degrees showed that chloroquine-sensitive mechanisms accounted for at least 50% of receptor-mediated insulin degradation in rat hepatocytes.
    [Abstract] [Full Text] [Related] [New Search]