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  • Title: lacZ--Y+ fusions in Escherichia coli. DNA sequencing reveals the eight N-terminal residues of lac permease as non-essential.
    Author: Bocklage H, Müller-Hill B.
    Journal: Eur J Biochem; 1983 Dec 15; 137(3):561-5. PubMed ID: 6363062.
    Abstract:
    The nucleotide sequence of three lacZ- -Y+ fusions found among spontaneous lacY+ revertants of the lacY- mutant MAB16 in Escherichia coli is reported. MAB16 is a frameshift mutation in codon 6 of the lacY gene. DNA sequence analysis of the fusions shows the first eight N-terminal codons of the lacY gene can be replaced by 3, 39 or 804/805 N-terminal codons of the lacZ gene without impairing lac permease activity qualitatively.
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