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  • Title: Immunophenotyping of leukaemia: an immunoperoxidase method using air-dried smears.
    Author: Sandhaus LM, Gajl-Peczalska KJ, Brunning RD.
    Journal: Br J Haematol; 1984 Jan; 56(1):131-8. PubMed ID: 6367805.
    Abstract:
    An immunoperoxidase (IP) method utilizing monoclonal antibodies to demonstrate the presence of the common acute lymphocytic leukaemia antigen (CALLA) on leukaemic cells using air-dried blood and bone marrow smears is described. Two readily available CALLA-specific monoclonal antibodies, BA-3 and J5, and an avidin-biotin immunoperoxidase methodology were utilized. Nineteen cases of acute leukaemia or blast crisis of chronic myelogenous leukaemia were studied. Immunofluorescent studies correlated closely with the results of the immunoperoxidase technique.
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