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  • Title: [Expression in Escherichia coli cells of mutant human interferon alpha2].
    Author: Ovchinnikov IuA, Sverdlov ED, Monastyrskaia GS, Tsarev SA, Zaĭtseva EM.
    Journal: Mol Biol (Mosk); 1984; 18(1):48-59. PubMed ID: 6369117.
    Abstract:
    cDNA library was obtained from mRNA isolated from human leukocytes induced by Newcastle disease virus. Clones containing cDNA for alpha 2-interferons were identified by colony hybridization with two synthetic hexadecanucleotides. One of the positive clones contained a NH2-terminal part of cDNA of human interferon identical to cDNA for IFN-alpha 2. The only difference between these two clones was the Ser-8 leads to Asn-8 substitution in deduced sequenced of mature interferons. This mutant interferon, named alpha 2, was expressed in E. coli and its properties were compared with those of interferon alpha 2.
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