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  • Title: Homogeneous enzyme immunoassay of diosgenin and its glycosides.
    Author: Sarkar M, Mahato SB.
    Journal: Anal Biochem; 1984 Jan; 136(1):168-74. PubMed ID: 6370034.
    Abstract:
    Homogeneous enzyme immunoassay has been used as a tool for the determination of diosgenin and its glycosides in plants. Diosgenin antisera was found to inhibit the activity of diosgenin hemisuccinate-horseradish peroxidase conjugate which was reversed by the addition of free diosgenin or its glycosides. The increase of enzyme activity was proportional to the quantity of the hapten over a certain range of hapten concentration. Thus, a minimum of 2.5 micrograms/ml of diosgenin and 11.5 micrograms/ml of diosgenin glycosides could be determined by this method. The results were comparable with those obtained by high-performance liquid chromatography and gravimetric methods.
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