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  • Title: An IFAT cercarial slide antigen preparation for schistosomiasis.
    Author: Azab ME, Safer EH, Ghaffar FM.
    Journal: Folia Parasitol (Praha); 1984; 31(1):93-6. PubMed ID: 6370803.
    Abstract:
    A modified technique for the preparation of a cercarial microscopic slide antigen for use in IFAT, that could be stored at -20 degrees C for 6 months is described. Cercariae were deposited by cooling, fixed in 0.5% buffered formaline, mixed with 7% egg albumin, dropped on slides and dried. Before use, antigen was post-fixed in absolute methyl alcohol. Evans blue was used as a counterstain. The antigen was evaluated with known human positive and negative sera, also with sera from normal individuals and others residing in endemic areas and exposed to infections with schistosomiasis; as well as with sera of patients with other helminthic infections (strongyloidiasis, ankylostomiasis, ascariasis and microfilariasis). It indicated a sensitivity of 83% and a specificity of 61.5%.
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