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  • Title: [Characterization of the subunits of botulinum neurotoxin type A].
    Author: Vinogradova ID, Uvarova RN, Baratova LA, Kazdobina IS, Ugriumova GA.
    Journal: Biokhimiia; 1984 Mar; 49(3):426-31. PubMed ID: 6372876.
    Abstract:
    A comparative amino acid analysis of botulinum neurotoxin type A and its subunits has been carried out. The heavy and light chains of neurotoxin have the same ratios of polar and non-polar amino acids (1.3:1), the amount of tryptophan residues in the heavy chain is 4 times as much as that in the light chain, and the number of SH-groups exceeds that in the light chains 2-fold. In neurotoxin, two N-terminal amino acid residues--alanine and leucine--were identified. Alanine was found to be the N-terminus of the heavy chain. The fluorescence spectra of neurotoxin subunits indicate differences in the conformational state of the polypeptide chains. The antigenic non-identity of botulinum neurotoxin A subunits suggests the presence in the neurotoxin molecule of at least two antigenic determinants, corresponding to the heavy and light chains.
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