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  • Title: [Dissociation of a Candida tropicalis culture and its capacity to stimulate levorin synthesis when cultured together with Actinomyces levoris].
    Author: Iakovleva EP, Sokolova EN.
    Journal: Antibiotiki; 1978 Mar; 23(3):199-203. PubMed ID: 637525.
    Abstract:
    Dissociation of the yeast-like fungi C. tropicalis used for mixed cultivation with the levorin-producing organism was studied. It was shown that colonies of 2 types were grown on wart-agar. They differed in the morphology and capacity for stimulation of the antiobiotic synthesis in mixed fermentation with 2 organisms. The colonies of the S-form increased the antibiotic production by 50--60 per cent, while the R-forms increased the antibiotic production only by 25--30 per cent. The strains of C. tropicalis differed in the ratio of the S- and R-forms in both the initial cultures and the cultures stored for prolonged periods of time. Addition of KH2PO4 to the agarized in the amount of 0.5 per cent promoted preservation of the S-forms in the population. Repeated subsequent transfers of the S-variants (10 generations) on wart-agar did not decrease the stimulating effect of C. tropicalis on the actinomycete. An increase in the amount of the inoculum of the yeast-like fungi promoted a decrease in the time of their preliminary cultivation.
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