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  • Title: Simple screening method for beta-lactamase-positive and -negative ampicillin-resistant Haemophilus influenzae isolates.
    Author: Parr TR, Pai CH, Bryan LE.
    Journal: J Clin Microbiol; 1984 Jul; 20(1):131-2. PubMed ID: 6378964.
    Abstract:
    A simple, inexpensive method for screening for beta-lactamase-positive and beta-lactamase-negative ampicillin-resistant Haemophilus influenzae isolates was developed. Disks containing 10 micrograms of cloxacillin yielded no zone of inhibition when placed on chocolate agar plates inoculated with beta-lactamase-positive (16 strains) or ampicillin-resistant (greater than or equal to 1 microgram/ml) beta-lactamase-negative (10 strains) H. influenzae, whereas ampicillin-susceptible (less than or equal to 0.5 microgram/ml; 36 strains) H. influenzae almost always (92%) showed a zone of inhibition.
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