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  • Title: Ultrasound-accelerated immunoassay, as exemplified by enzyme immunoassay of choriogonadotropin.
    Author: Chen R, Weng L, Sizto NC, Osorio B, Hsu CJ, Rodgers R, Litman DJ.
    Journal: Clin Chem; 1984 Sep; 30(9):1446-51. PubMed ID: 6380810.
    Abstract:
    The rate-limiting step in many solid-phase immunoassays is associated with the slow kinetics of binding of macro-molecular antigen and conjugate to the immobilized phase. We demonstrate that the use of ultrasonic energy to enhance mass transport across liquid/solid interfaces can dramatically accelerate antigen binding to immobilized antibodies. We use an ultrasound-accelerated procedure with an enzyme-channelling test strip containing glucose oxidase and specific antibody to the alpha-subunit of human choriogonadotropin (HCG) co-immobilized onto a cellulose support. A horseradish peroxidase conjugate of monospecific antibody to the beta-subunit of HCG is used in the liquid phase to complete the immune "sandwich." Use of ultrasound to accelerate binding and of enzyme channelling to eliminate wash steps result in a simple two-incubation protocol by which 25 int. units of urinary HCG per liter can be detected visually in less than 20 min of assay time.
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