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  • Title: Serotonin immunohistochemistry reveals immature EC cells.
    Author: Inokuchi H, Azuma T, Kawai K, Takeuchi Y, Sano Y.
    Journal: Histochemistry; 1984; 80(5):517-8. PubMed ID: 6384153.
    Abstract:
    Radioautographic studies of rat duodenal mucosa were carried out after single injections of 3H-thymidine, and the labelling indices of enterochromaffin (EC) cells identified by serotonin immunohistochemistry and by the diazo reaction were compared. The labelling index of EC cells by serotonin immunohistochemistry was 2.3% (mean percentage of 2 rats), while no labelled cells were found in diazo-positive EC cells. This finding indicates that serotonin immunohistochemistry reveals a less mature population of EC cells than conventional techniques such as the diazo reaction.
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