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  • Title: Immunoelectronmicroscopic profile of intracytoplasmic immunoglobulins in B non Hodgkin's malignant lymphomas. Comparison with the normal pattern of B lymphoid cells.
    Author: Reynes M, Tricottet V, Capron F, Jaafoura MH, Galtier M, Diebold J.
    Journal: Pathol Res Pract; 1984 Jul; 178(6):570-8. PubMed ID: 6384972.
    Abstract:
    An immunoelectronmicroscopic method using Fab fragment of anti human IgG (H + L) has been employed to study the localization of cytoplasmic immunoglobulins in the tumoral cells of 12 B non Hodgkin's malignant lymphomas (B-M.L.). A comparison with normal homologous B lymphoid cells was performed on 10 non tumoral reactive lymph nodes. Immunostaining was observed in PNC, RER and Golgi complex. The criterions of differentiation were discussed in the different B-M.L.. Because of a granular hyaloplasmic immunostaining in normal and tumoral centroblasts and immunoblasts, monospecific antibodies against gamma, mu, alpha heavy chains were used to rule out a non specific uptake. Presence of mu heavy chain was discussed as an argument for immunoglobulin free ribosomal synthesis.
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