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  • Title: The measurement of urinary LH, by a solid-phase chemiluminescence immunoassay.
    Author: Brockelbank JL, Kim JB, Barnard GJ, Collins WP, Gaier B, Kohen F.
    Journal: Ann Clin Biochem; 1984 Jul; 21 ( Pt 4)():284-9. PubMed ID: 6385812.
    Abstract:
    Ovulation in women may be predicted or detected by a simple, solid-phase, chemiluminescence immunoassay for the measurement of urinary LH. An IgG fraction of a donkey antiserum to rabbit immunoglobulins is passively adsorbed to the walls of polystyrene tubes. Human chorionic gonadotrophin-succinyl-butyl-ethyl-isoluminol and a primary antibody (rabbit anti-human LH) is incubated with samples of early morning urine. After the binding reaction, the solution is removed by aspiration. The antibody-bound fraction is washed twice with buffer. Sodium hydroxide is added and the mixture incubated. After cooling, luminescence is initiated by oxidation of the label with microperoxidase/hydrogen peroxide and the signal integrated. The method has been evaluated in terms of sensitivity, within- and between-batch precision, bias and parallelism. The time interval between the peak day of LH in EMU and maximum follicular diameter during 38 menstrual cycles was -24 h (11%), O h (50%), + 24 h (28%) and + 48 h (11%).
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