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  • Title: Ultrastructural localization of carcino-embryonic antigen in a continuous human tumour cell line (LS 174 T) in relation to morphometric investigations.
    Author: Wolf B, Thompson J, Von Kleist S.
    Journal: Anticancer Res; 1984; 4(4-5):213-9. PubMed ID: 6385822.
    Abstract:
    The synthesis of carcinoembryonic antigen (CEA) in human tumour cell cultures has been investigated at the ultrastructural level in the colon carcinoma cell line LS 174 T. By application of a CEA-specific antibody fraction, gained from goat serum which was directly coupled with horse radish peroxidase (HRP), the expression of CEA could be followed in the cell culture both intra- and extracellularly with ultra-immunohistochemical methods, utilizing the pre-embedding technique. Morphometric and ultra-immunohistochemical investigations showed the possible involvement of the Golgi systems in CEA production. The changes in the volume fraction of the Golgi apparatus related to the total cell volume showed a direct correlation with the CEA content in the cell culture supernatants. Freeze-etch control experiments revealed negligible structural changes in the cells following ethyl-dimethyl-aminopropylcarbodiimide (EDC)-and saponin permeation techniques.
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