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  • Title: Facilitated diffusion of monosaccharides in smooth muscle of rat vas deferens in vitro.
    Author: Elbrink J.
    Journal: Can J Physiol Pharmacol; 1984 Sep; 62(9):1112-5. PubMed ID: 6388766.
    Abstract:
    The suitability of rat vas deferens for investigating sugar transport in smooth muscle was determined in vitro, with the nonmetabolized glucose analog 3-O-methyl-D-glucose as test sugar. Vas deferens smooth muscle contains a facilitated diffusion system for monosaccharides, as shown by saturation of the transport sites and by competition between 3-O-methyl-D-glucose and D-glucose. The activity of the facilitated diffusion system could be enhanced by hyperosmolarity and by contractile activity, but frequency dependency could not be established. A high concentration of insulin (100 mU/mL) was required to stimulate sugar transport. As smooth muscle is not a primary tissue for the storage of energy reserves, it does not require large numbers of insulin receptors.
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